Translational_Unit

Part:BBa_K3522010:Design

Designed by: Kong Yangyang   Group: iGEM20_NOFLS_YZ   (2020-10-17)


CMV-RXRα-BGH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1308
    Illegal BamHI site found at 1461
    Illegal BamHI site found at 1575
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1873
    Illegal AgeI site found at 1161
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To meet the type IIS standard, we made a synonymous mutation of Thr280Thr to remove the BsaI restriction site. Apart from that, we design an SV40 enhancer located at the front of the SV40 promoter to express RXRα better.


Source

NDB stabilizes hFXRα homodimerization.

References